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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
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- Product number
- AF2974 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TFPI Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TFPI in direct ELISAs and Western blots. In directs ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) TFPI is observed and less than 1% cross-reactivity with recombinant human TFPI-2, and rmTFPI-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells.
Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies.
Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
Wiley CD, Liu S, Limbad C, Zawadzka AM, Beck J, Demaria M, Artwood R, Alimirah F, Lopez-Dominguez JA, Kuehnemann C, Danielson SR, Basisty N, Kasler HG, Oron TR, Desprez PY, Mooney SD, Gibson BW, Schilling B, Campisi J, Kapahi P
Cell reports 2019 Sep 24;28(13):3329-3337.e5
Cell reports 2019 Sep 24;28(13):3329-3337.e5
Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies.
Slatter DA, Percy CL, Allen-Redpath K, Gajsiewicz JM, Brooks NJ, Clayton A, Tyrrell VJ, Rosas M, Lauder SN, Watson A, Dul M, Garcia-Diaz Y, Aldrovandi M, Heurich M, Hall J, Morrissey JH, Lacroix-Desmazes S, Delignat S, Jenkins PV, Collins PW, O'Donnell VB
JCI insight 2018 Mar 22;3(6)
JCI insight 2018 Mar 22;3(6)
Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
Molecular & cellular proteomics : MCP 2008 Oct;7(10):1974-82
Molecular & cellular proteomics : MCP 2008 Oct;7(10):1974-82
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TFPI by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFPI at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TFPI by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TFPI at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
