Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB3223 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3223, RRID:AB_10559014
- Product name
- Itgam monoclonal antibody, clone 3A33
- Antibody type
- Monoclonal
- Antigen
- Native Itgam from mouse peritoneal macrophages.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 500 µg
- Storage
- Store at 4°C.
Submitted references Markers of mouse macrophage development detected by monoclonal antibodies.
Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm.
The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells.
Leenen PJ, de Bruijn MF, Voerman JS, Campbell PA, van Ewijk W
Journal of immunological methods 1994 Sep 14;174(1-2):5-19
Journal of immunological methods 1994 Sep 14;174(1-2):5-19
Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm.
Springer TA
Cell 1994 Jan 28;76(2):301-14
Cell 1994 Jan 28;76(2):301-14
The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells.
Vremec D, Zorbas M, Scollay R, Saunders DJ, Ardavin CF, Wu L, Shortman K
The Journal of experimental medicine 1992 Jul 1;176(1):47-58
The Journal of experimental medicine 1992 Jul 1;176(1):47-58
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
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- Experimental details
- BALB/c splenocytes were incubated with Itgam monoclonal antibody, clone 3A33 (Cat # MAB3223) and then stained with goat anti-rat IgG (H+L)-FITC. Large cells were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
- Validation comment
- Flow Cytometry