MAB3224

antibody from Abnova Corporation

Targeting: ITGAM CD11B, CR3A, MAC-1

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3224

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB3224, RRID:AB_10556158

Product name

Itgam monoclonal antibody, clone M1/70

Antibody type

Monoclonal

Antigen

Native Itgam from mouse splenic T cells and splenocytes.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

500 µg

Storage

Store at 4°C.

References

Submitted references

Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection.

Gyetko MR, Sud S, Kendall T, Fuller JA, Newstead MW, Standiford TJ
Journal of immunology (Baltimore, Md. : 1950) 2000 Aug 1;165(3):1513-9

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Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54).

Lub M, van Kooyk Y, Figdor CG
Journal of leukocyte biology 1996 May;59(5):648-55

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Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm.

Springer TA
Cell 1994 Jan 28;76(2):301-14

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BALB/c splenocytes were double stained with Itgam monoclonal antibody, clone M1/70 (Cat # MAB3224)-FITC and rat anti-mouse Ly-6G-PE. Lymphocytes were gated and analyzed by flow cytometry.

Validation comment

Flow Cytometry