M00144

antibody from Boster Biological Technology

Targeting: ITGAM CD11B, CR3A, MAC-1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00144

Provider

Boster Biological Technology

Product name

Anti-CD11b ITGAM Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for CD11b/ITGAM detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human CD11b/ITGAM information: Molecular Weight: 127179 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Predominantly expressed in monocytes and granulocytes.

Reactivity

Human

Host

Rabbit

Antibody clone number

BCG-9

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody(M00144)ITGAM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGAM Antibody (M00144)overnight at 4

Additional image