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- Antibody Data
- Antigen structure
- References [28]
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- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB414-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse G-CSF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse G-CSF in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) G-CSF, rhCNTF, rmIL-6, rmLIF, or rmOSM.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P09920- Isotype
- IgG
- Antibody clone number
- 67604
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Modulating Bone Marrow Hematopoietic Lineage Potential to Prevent Bone Metastasis in Breast Cancer.
Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection.
Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance.
Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils.
Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis.
An NK Cell Perforin Response Elicited via IL-18 Controls Mucosal Inflammation Kinetics during Salmonella Gut Infection.
Depletion of CD11c⁺ cells in the CD11c.DTR model drives expansion of unique CD64⁺ Ly6C⁺ monocytes that are poised to release TNF-α.
Tumor-secreted products repress B-cell lymphopoiesis in a murine model of breast cancer.
Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis.
The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury.
IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils.
Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
IL-17-producing γδ T cells and neutrophils conspire to promote breast cancer metastasis.
Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals.
Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1α/IL-6 loop.
G-CSF drives a posttraumatic immune program that protects the host from infection.
Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Inflammation triggers emergency granulopoiesis through a density-dependent feedback mechanism.
Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models.
Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow.
The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse.
The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
Ubellacker JM, Baryawno N, Severe N, DeCristo MJ, Sceneay J, Hutchinson JN, Haider MT, Rhee CS, Qin Y, Gregory WM, Garrido-Castro AC, Holen I, Brown JE, Coleman RE, Scadden DT, McAllister SS
Cancer research 2018 Sep 15;78(18):5300-5314
Cancer research 2018 Sep 15;78(18):5300-5314
Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection.
Sparber F, Dolowschiak T, Mertens S, Lauener L, Clausen BE, Joller N, Stoitzner P, Tussiwand R, LeibundGut-Landmann S
PLoS pathogens 2018 May;14(5):e1007069
PLoS pathogens 2018 May;14(5):e1007069
Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance.
Meyer MA, Baer JM, Knolhoff BL, Nywening TM, Panni RZ, Su X, Weilbaecher KN, Hawkins WG, Ma C, Fields RC, Linehan DC, Challen GA, Faccio R, Aft RL, DeNardo DG
Nature communications 2018 Mar 28;9(1):1250
Nature communications 2018 Mar 28;9(1):1250
Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils.
Banuelos J, Cao Y, Shin SC, Bochner BS, Avila P, Li S, Jiang X, Lingen MW, Schleimer RP, Lu NZ
PloS one 2017;12(5):e0177884
PloS one 2017;12(5):e0177884
Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis.
Zhu H, Kwak HJ, Liu P, Bajrami B, Xu Y, Park SY, Nombela-Arrieta C, Mondal S, Kambara H, Yu H, Chai L, Silberstein LE, Cheng T, Luo HR
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2854-2864
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2854-2864
An NK Cell Perforin Response Elicited via IL-18 Controls Mucosal Inflammation Kinetics during Salmonella Gut Infection.
Müller AA, Dolowschiak T, Sellin ME, Felmy B, Verbree C, Gadient S, Westermann AJ, Vogel J, LeibundGut-Landmann S, Hardt WD
PLoS pathogens 2016 Jun;12(6):e1005723
PLoS pathogens 2016 Jun;12(6):e1005723
Depletion of CD11c⁺ cells in the CD11c.DTR model drives expansion of unique CD64⁺ Ly6C⁺ monocytes that are poised to release TNF-α.
Sivakumaran S, Henderson S, Ward S, Sousa PS, Manzo T, Zhang L, Conlan T, Means TK, D'Aveni M, Hermine O, Rubio MT, Chakraverty R, Bennett CL
European journal of immunology 2016 Jan;46(1):192-203
European journal of immunology 2016 Jan;46(1):192-203
Tumor-secreted products repress B-cell lymphopoiesis in a murine model of breast cancer.
Moreau JM, Mielnik M, Berger A, Furlonger C, Paige CJ
European journal of immunology 2016 Dec;46(12):2835-2841
European journal of immunology 2016 Dec;46(12):2835-2841
Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis.
Campbell IK, Leong D, Edwards KM, Rayzman V, Ng M, Goldberg GL, Wilson NJ, Scalzo-Inguanti K, Mackenzie-Kludas C, Lawlor KE, Wicks IP, Brown LE, Baz Morelli A, Panousis C, Wilson MJ, Nash AD, McKenzie BS, Andrews AE
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4392-4402
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4392-4402
The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Damgaard RB, Walker JA, Marco-Casanova P, Morgan NV, Titheradge HL, Elliott PR, McHale D, Maher ER, McKenzie ANJ, Komander D
Cell 2016 Aug 25;166(5):1215-1230.e20
Cell 2016 Aug 25;166(5):1215-1230.e20
Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury.
Bohannon JK, Luan L, Hernandez A, Afzal A, Guo Y, Patil NK, Fensterheim B, Sherwood ER
Journal of leukocyte biology 2016 Apr;99(4):629-40
Journal of leukocyte biology 2016 Apr;99(4):629-40
IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils.
Trautwein-Weidner K, Gladiator A, Nur S, Diethelm P, LeibundGut-Landmann S
Mucosal immunology 2015 Mar;8(2):221-31
Mucosal immunology 2015 Mar;8(2):221-31
Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
Brooks R, Iyer S, Akada H, Neelam S, Russo CM, Chisholm JD, Kerr WG
Stem cells (Dayton, Ohio) 2015 Mar;33(3):848-58
Stem cells (Dayton, Ohio) 2015 Mar;33(3):848-58
IL-17-producing γδ T cells and neutrophils conspire to promote breast cancer metastasis.
Coffelt SB, Kersten K, Doornebal CW, Weiden J, Vrijland K, Hau CS, Verstegen NJM, Ciampricotti M, Hawinkels LJAC, Jonkers J, de Visser KE
Nature 2015 Jun 18;522(7556):345-348
Nature 2015 Jun 18;522(7556):345-348
Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals.
Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson AK
Cancer research 2015 Jul 1;75(13):2653-62
Cancer research 2015 Jul 1;75(13):2653-62
Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1α/IL-6 loop.
Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto KI, Simmons O, Toda S, Conway SJ, Hamasaki Y, Izuhara K
The Journal of investigative dermatology 2014 May;134(5):1295-1304
The Journal of investigative dermatology 2014 May;134(5):1295-1304
G-CSF drives a posttraumatic immune program that protects the host from infection.
Gardner JC, Noel JG, Nikolaidis NM, Karns R, Aronow BJ, Ogle CK, McCormack FX
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2405-17
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2405-17
Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure.
Kulkarni S, Singh PK, Ghosh SP, Posarac A, Singh VK
Cytokine 2013 May;62(2):278-85
Cytokine 2013 May;62(2):278-85
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Inflammation triggers emergency granulopoiesis through a density-dependent feedback mechanism.
Cain DW, Snowden PB, Sempowski GD, Kelsoe G
PloS one 2011;6(5):e19957
PloS one 2011;6(5):e19957
Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
Waight JD, Hu Q, Miller A, Liu S, Abrams SI
PloS one 2011;6(11):e27690
PloS one 2011;6(11):e27690
Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
The Journal of clinical investigation 2011 Nov;121(11):4446-61
The Journal of clinical investigation 2011 Nov;121(11):4446-61
G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models.
Shojaei F, Wu X, Qu X, Kowanetz M, Yu L, Tan M, Meng YG, Ferrara N
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 21;106(16):6742-7
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 21;106(16):6742-7
Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow.
Brunner S, Zaruba MM, Huber B, David R, Vallaster M, Assmann G, Mueller-Hoecker J, Franz WM
Experimental hematology 2008 Sep;36(9):1157-66
Experimental hematology 2008 Sep;36(9):1157-66
The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse.
Furze RC, Rankin SM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Sep;22(9):3111-9
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Sep;22(9):3111-9
The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
Wengner AM, Pitchford SC, Furze RC, Rankin SM
Blood 2008 Jan 1;111(1):42-9
Blood 2008 Jan 1;111(1):42-9
Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
Nature 2007 Dec 6;450(7171):825-31
Nature 2007 Dec 6;450(7171):825-31
Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK
The Journal of experimental medicine 2001 Aug 20;194(4):519-27
The Journal of experimental medicine 2001 Aug 20;194(4):519-27
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of G-CSF in Mouse splenocytes by Flow Cytometry. Mouse splenocytes treated with LPS (100 μg/ml) for 24 hours were stained with Rat Anti-Mouse G-CSF Monoclonal Antibody (Catalog # MAB414, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by Anti-Rat IgG PE-conjugated secondary antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by G-CSF and Neutralization by Mouse G-CSF Antibody. Recombinant Mouse G-CSF (Catalog # 414-CS) stimulates proliferation in the NFS-60 mouse myeloid cells line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse G-CSF Monoclonal Antibody (Catalog # MAB414). The ND50 is typically 0.01-0.03 µg/mL.
