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- Antibody Data
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- References [2]
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- Product number
- AF-414-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse G-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse G-CSF in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant human G-CSF is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P09920- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.
Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
Shimoji K, Yuasa S, Onizuka T, Hattori F, Tanaka T, Hara M, Ohno Y, Chen H, Egasgira T, Seki T, Yae K, Koshimizu U, Ogawa S, Fukuda K
Cell stem cell 2010 Mar 5;6(3):227-37
Cell stem cell 2010 Mar 5;6(3):227-37
Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
Nature 2007 Dec 6;450(7171):825-31
Nature 2007 Dec 6;450(7171):825-31
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mouse G-CSF ELISA Standard Curve. Recombinant Mouse G-CSF protein was serially diluted 2-fold and captured by Rat Anti-Mouse G-CSF Monoclonal Antibody (Catalog # MAB414R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by G-CSF and Neutralization by Mouse G-CSF Antibody. Recombinant Mouse G-CSF (Catalog # 414-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA). The ND50 is typically 0.0025-0.0075 µg/mL.
