500-P43-100UG

antibody from Invitrogen Antibodies

Targeting: CSF3 C17orf33, G-CSF, GCSF, MGC45931

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P43-100UG

Provider

Invitrogen Antibodies

Product name

G-CSF Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP.

Concentration

0.1-1.0 mg/mL

References

Submitted references

Ubiquitin fusion expression and tissue-dependent targeting of hG-CSF in transgenic tobacco.

Tian L, Sun SS
BMC biotechnology 2011 Oct 11;11:91

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G-CSF down-regulation of CXCR4 expression identified as a mechanism for mobilization of myeloid cells.

Kim HK, De La Luz Sierra M, Williams CK, Gulino AV, Tosato G
Blood 2006 Aug 1;108(3):812-20

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hG-CSF by Western Blot analysis G-CSF Polyclonal Antibody (Product # 500-P43-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hG-CSF by Western Blot analysis G-CSF Polyclonal Antibody (Product # 500-P43-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human G-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of G-CSF Polyclonal Antibody (Product # 500-P43-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech G-CSF Polyclonal Antibody, Biotin (Product # 500-P43BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human G-CSF.