TA336452

antibody from OriGene

Targeting: CHUK IkBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA336452

Provider

OriGene

Product name

Mouse Monoclonal IKK alpha Antibody (14A231)

Antibody type

Monoclonal

Description

Mouse Monoclonal IKK alpha Antibody (14A231)

Host

Mouse

Conjugate

Unconjugated

Epitope

CHUK

Isotype

IgG

Antibody clone number

14A231

Vial size

100 µg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: IKK alpha Antibody (14A231) [TA336452] - analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/ml.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [TA336452] - IKK alpha antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilutio

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) [TA336452] - Formalin-fixed, paraffin-embedded human kidney (IMH-343) stained with IKK alpha antibody at 5 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow Cytometry: IKK alpha Antibody (14A231) [TA336452] - Intracellular staining of HEK293 cells using 0.5 ug of IKK alpha antibody. Green histogram represents the isotype control antibody, red represents the IKK alpha antibody. Intracellular flow kit was

Validation comment

FC