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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ARG65746 - Provider product page
- Provider
- Arigo
- Product name
- anti-IKK alpha antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around the N-terminus of Human IKK alpha (NP_001269.3).
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of H1299 stained with ARG65746 anti-IKK alpha antibody at 1:500 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: N2A cells stained with ARG65746 anti-IKK alpha antibody. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human mammary gland tissue stained with ARG65746 anti-IKK alpha antibody. Counter stained with hematoxylin.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human gastric carcinoma tissue stained with ARG65746 anti-IKK alpha antibody. Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG65746 anti-IKK alpha antibody at 1:100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
