H00001147-M03

antibody from Abnova Corporation

Targeting: CHUK IkBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001147-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001147-M03, RRID:AB_534817

Product name

CHUK monoclonal antibody (M03), clone 4B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CHUK.

Antigen sequence

RQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTS
AWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLN
CLGHLSTIIHEANEEQGNSMMNLDWSWLTE


Isotype

IgG

Antibody clone number

4B8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CHUK monoclonal antibody (M03), clone 4B8 Western Blot analysis of CHUK expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CHUK is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol