H00001147-M04

antibody from Invitrogen Antibodies

Targeting: CHUK IkBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001147-M04

Provider

Invitrogen Antibodies

Product name

CHUK Monoclonal Antibody (2G4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: RQKEIWHLLK IACTQSSARS LVGSSLEGAV TPQTSAWLPP TSAEHDHSLS CVVTPQDGET SAQMIEENLN CLGHLSTIIH EANEEQGNSM MNLDWSWLTE

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2G4

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CHUK monoclonal antibody (M04), clone 2G4 Western Blot analysis of CHUK expression in Jurkat (Cat # L017V1).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. Antibody concentration is 10 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human placenta. Antibody concentration is 3 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between IKBKB and CHUK. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).