Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [2]
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Validation data
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- Product number
- NB100-56513 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56513, RRID:AB_838414
- Product name
- Mouse Monoclonal IKK beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. An approx. 87 kDa band should be observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references T cell receptor signals to NF-κB are transmitted by a cytosolic p62-Bcl10-Malt1-IKK signalosome.
IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.
KU812 cells provide a novel in vitro model of the human IL-33/ST2L axis: functional responses and identification of signaling pathways.
CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis.
Notch1 upregulates LPS-induced macrophage activation by increasing NF-kappaB activity.
The RING domain and first zinc finger of TRAF6 coordinate signaling by interleukin-1, lipopolysaccharide, and RANKL.
Beta2-integrin-induced p38 MAPK activation is a key mediator in the CD14/TLR4/MD2-dependent uptake of lipopolysaccharide by hepatocytes.
Notch3 and pre-TCR interaction unveils distinct NF-kappaB pathways in T-cell development and leukemia.
IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway.
Paul S, Traver MK, Kashyap AK, Washington MA, Latoche JR, Schaefer BC
Science signaling 2014 May 13;7(325):ra45
Science signaling 2014 May 13;7(325):ra45
IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.
Bakkar N, Ladner K, Canan BD, Liyanarachchi S, Bal NC, Pant M, Periasamy M, Li Q, Janssen PM, Guttridge DC
The Journal of cell biology 2012 Feb 20;196(4):497-511
The Journal of cell biology 2012 Feb 20;196(4):497-511
KU812 cells provide a novel in vitro model of the human IL-33/ST2L axis: functional responses and identification of signaling pathways.
Tare N, Li H, Morschauser A, Cote-Sierra J, Ju G, Renzetti L, Lin TA
Experimental cell research 2010 Sep 10;316(15):2527-37
Experimental cell research 2010 Sep 10;316(15):2527-37
CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis.
Yang G, Rosen DG, Liu G, Yang F, Guo X, Xiao X, Xue F, Mercado-Uribe I, Huang J, Lin SH, Mills GB, Liu J
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Aug 1;16(15):3875-86
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Aug 1;16(15):3875-86
Notch1 upregulates LPS-induced macrophage activation by increasing NF-kappaB activity.
Monsalve E, Ruiz-García A, Baladrón V, Ruiz-Hidalgo MJ, Sánchez-Solana B, Rivero S, García-Ramírez JJ, Rubio A, Laborda J, Díaz-Guerra MJ
European journal of immunology 2009 Sep;39(9):2556-70
European journal of immunology 2009 Sep;39(9):2556-70
The RING domain and first zinc finger of TRAF6 coordinate signaling by interleukin-1, lipopolysaccharide, and RANKL.
Lamothe B, Campos AD, Webster WK, Gopinathan A, Hur L, Darnay BG
The Journal of biological chemistry 2008 Sep 5;283(36):24871-80
The Journal of biological chemistry 2008 Sep 5;283(36):24871-80
Beta2-integrin-induced p38 MAPK activation is a key mediator in the CD14/TLR4/MD2-dependent uptake of lipopolysaccharide by hepatocytes.
Scott MJ, Billiar TR
The Journal of biological chemistry 2008 Oct 24;283(43):29433-46
The Journal of biological chemistry 2008 Oct 24;283(43):29433-46
Notch3 and pre-TCR interaction unveils distinct NF-kappaB pathways in T-cell development and leukemia.
Vacca A, Felli MP, Palermo R, Di Mario G, Calce A, Di Giovine M, Frati L, Gulino A, Screpanti I
The EMBO journal 2006 Mar 8;25(5):1000-8
The EMBO journal 2006 Mar 8;25(5):1000-8
IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway.
Fitzgerald KA, McWhirter SM, Faia KL, Rowe DC, Latz E, Golenbock DT, Coyle AJ, Liao SM, Maniatis T
Nature immunology 2003 May;4(5):491-6
Nature immunology 2003 May;4(5):491-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Antibodoy was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. IKKb was detected as a protein of ~87 kD.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56513. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control, red represents the IKK antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control , red represents the IKK antibody. Image using the Azide Free format of this antibody.