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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF4535 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IKK beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human IKK beta in Western blots. In Western blots, this antibody does not cross-react with recombinant human IKK alpha , IKK gamma , or IKK epsilon .
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O14920- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages.
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.
Awuh JA, Haug M, Mildenberger J, Marstad A, Do CP, Louet C, Stenvik J, Steigedal M, Damås JK, Halaas Ø, Flo TH
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 4;112(31):E4272-80
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 4;112(31):E4272-80
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.
Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
PloS one 2014;9(6):e100787
PloS one 2014;9(6):e100787
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human IKK beta by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human IKK beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4535) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
