Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1619 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-23 p19 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL-12/23 p40 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9EQ14- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation.
IL-17A Produced by Innate Lymphoid Cells Is Essential for Intestinal Ischemia-Reperfusion Injury.
Dendritic Cells in Irradiated Mice Trigger the Functional Plasticity and Antitumor Activity of Adoptively Transferred Tc17 Cells via IL12 Signaling.
Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
RORγt+ innate lymphoid cells regulate intestinal homeostasis by integrating negative signals from the symbiotic microbiota.
Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease.
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Lack of Toll IL-1R8 exacerbates Th17 cell responses in fungal infection.
Lack of Toll IL-1R8 exacerbates Th17 cell responses in fungal infection.
Tumor-specific Th17-polarized cells eradicate large established melanoma.
TLR3 and TLR7 are involved in expression of IL-23 subunits while TLR3 but not TLR7 is involved in expression of IFN-beta by Theiler's virus-infected RAW264.7 cells.
Epicutaneous antigen exposure induces a Th17 response that drives airway inflammation after inhalation challenge.
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo.
Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo.
Marks E, Naudin C, Nolan G, Goggins BJ, Burns G, Mateer SW, Latimore JK, Minahan K, Plank M, Foster PS, Callister R, Veysey M, Walker MM, Talley NJ, Radford-Smith G, Keely S
Mucosal immunology 2017 Sep;10(5):1224-1236
Mucosal immunology 2017 Sep;10(5):1224-1236
IL-17A Produced by Innate Lymphoid Cells Is Essential for Intestinal Ischemia-Reperfusion Injury.
Geha M, Tsokos MG, Bosse RE, Sannikova T, Iwakura Y, Dalle Lucca JJ, De Waal Malefyt R, Tsokos GC
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 15;199(8):2921-2929
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 15;199(8):2921-2929
Dendritic Cells in Irradiated Mice Trigger the Functional Plasticity and Antitumor Activity of Adoptively Transferred Tc17 Cells via IL12 Signaling.
Bowers JS, Nelson MH, Kundimi S, Bailey SR, Huff LW, Schwartz KM, Cole DJ, Rubinstein MP, Paulos CM
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jun 1;21(11):2546-57
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jun 1;21(11):2546-57
Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
Infection and immunity 2012 Jan;80(1):410-7
Infection and immunity 2012 Jan;80(1):410-7
Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesnière A, Ibrahim N, Déchanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
The Journal of experimental medicine 2011 Mar 14;208(3):491-503
The Journal of experimental medicine 2011 Mar 14;208(3):491-503
The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
El-Behi M, Ciric B, Dai H, Yan Y, Cullimore M, Safavi F, Zhang GX, Dittel BN, Rostami A
Nature immunology 2011 Jun;12(6):568-75
Nature immunology 2011 Jun;12(6):568-75
The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):869-77
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):869-77
RORγt+ innate lymphoid cells regulate intestinal homeostasis by integrating negative signals from the symbiotic microbiota.
Sawa S, Lochner M, Satoh-Takayama N, Dulauroy S, Bérard M, Kleinschek M, Cua D, Di Santo JP, Eberl G
Nature immunology 2011 Apr;12(4):320-6
Nature immunology 2011 Apr;12(4):320-6
Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease.
Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ
Nature medicine 2010 Feb;16(2):191-7
Nature medicine 2010 Feb;16(2):191-7
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA Jr, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Lack of Toll IL-1R8 exacerbates Th17 cell responses in fungal infection.
Bozza S, Zelante T, Moretti S, Bonifazi P, DeLuca A, D'Angelo C, Giovannini G, Garlanda C, Boon L, Bistoni F, Puccetti P, Mantovani A, Romani L
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4022-31
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4022-31
Lack of Toll IL-1R8 exacerbates Th17 cell responses in fungal infection.
Bozza S, Zelante T, Moretti S, Bonifazi P, DeLuca A, D'Angelo C, Giovannini G, Garlanda C, Boon L, Bistoni F, Puccetti P, Mantovani A, Romani L
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4022-31
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4022-31
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood 2008 Jul 15;112(2):362-73
Blood 2008 Jul 15;112(2):362-73
TLR3 and TLR7 are involved in expression of IL-23 subunits while TLR3 but not TLR7 is involved in expression of IFN-beta by Theiler's virus-infected RAW264.7 cells.
Al-Salleeh F, Petro TM
Microbes and infection 2007 Sep;9(11):1384-92
Microbes and infection 2007 Sep;9(11):1384-92
Epicutaneous antigen exposure induces a Th17 response that drives airway inflammation after inhalation challenge.
He R, Oyoshi MK, Jin H, Geha RS
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 2;104(40):15817-22
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 2;104(40):15817-22
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1341-8
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1341-8
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1341-8
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1341-8
Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo.
Hajishengallis G, Shakhatreh MA, Wang M, Liang S
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 15;179(4):2359-67
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 15;179(4):2359-67
Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo.
Hajishengallis G, Shakhatreh MA, Wang M, Liang S
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 15;179(4):2359-67
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 15;179(4):2359-67
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-17 secretion Induced by IL-23 and Neutralization by Mouse IL-23 Antibody. In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Mouse IL-23 (Catalog # 1887-ML) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Mouse IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1619). The ND50 is typically 0.07-0.35 µg/mL.
