PA5-20239

antibody from Invitrogen Antibodies

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20239

Provider

Invitrogen Antibodies

Product name

IL-23 p19 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is mouse pancreas tissue lysate.

Concentration

1 mg/mL

References

Submitted references

Primary alterations during the development of hidradenitis suppurativa.

Dajnoki Z, Somogyi O, Medgyesi B, Jenei A, Szabó L, Gáspár K, Hendrik Z, Gergely P, Imre D, Póliska S, Törőcsik D, Zouboulis CC, Prens EP, Kapitány A, Szegedi A
Journal of the European Academy of Dermatology and Venereology : JEADV 2022 Mar;36(3):462-471

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Spontaneous pulmonary hypertension in genetic mouse models of natural killer cell deficiency.

Rätsep MT, Moore SD, Jafri S, Mitchell M, Brady HJM, Mandelboim O, Southwood M, Morrell NW, Colucci F, Ormiston ML
American journal of physiology. Lung cellular and molecular physiology 2018 Dec 1;315(6):L977-L990

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IL23 and TGF-ß diminish macrophage associated metastasis in pancreatic carcinoma.

Hussain SM, Reed LF, Krasnick BA, Miranda-Carboni G, Fields RC, Bi Y, Elahi A, Ajidahun A, Dickson PV, Deneve JL, Hawkins WG, Shibata D, Glazer ES
Scientific reports 2018 Apr 11;8(1):5808

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse pancreas tissue lysate using a IL-23 polyclonal antibody (Product # PA5-20239) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Mouse Pancreas Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20239) (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Mouse Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20239) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-23 recombinant protein per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20239) (1: 0.25 µg/mL, 2: 0.5 µg/mL, 3: 1 µg/mL, and 4: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling IL-23 with IL-23 p19 Polyclonal Antibody (Product # PA5-20239) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using IL-23 p19 Polyclonal Antibody (Product # PA5-20239) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6. Increased IL-23 production and downstream cytokine expression in the Ncr1-Gfp and Nfil3 -/- mouse models of natural killer cell impairment and deficiency. Assessment of inflammatory gene expression in the lungs of Ncr1 +/+ , Ncr1 +/gfp , and Ncr1 gfp/gfp mice ( A ), normalized to the Rpl32 reference gene ( n = 4-11; 1-way ANOVA, Dunnett's posttest vs. Ncr1 +/+ controls). Representative immunofluorescent staining ( B ) of mouse lung tissue for IL-23 (magenta), macrophages (F4/80, yellow), and nuclei (DAPI, blue). Scale bar = 50 mum. Quantification of IL-23 + cells ( C and G ), F4/80 + macrophages ( D and H ), IL-23 + macrophages ( E and I ), and IL-23 + F4/80 - cells ( F and J ) in the lungs of both Ncr1-Gfp mice ( C - F ) ( n = 5-6; 1-way ANOVA, Dunnett's posttest vs. Ncr1 +/+ controls) and Nfil3 -/- mice ( G - J ) with pulmonary hypertension or WT littermate controls ( n = 6-13; Student's t -test). Expression of Th17-associated cytokines ( K ) in the lungs of Nfil3 -/- mice with pulmonary hypertension and WT littermate controls ( n = 5-13; Student's t -test). * P < 0.05 and ** P < 0.01. Means +- SE.