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Learnsc-32922
antibody from Santa Cruz Biotechnology
Targeting: VPS4A
FLJ22197, SKD1, SKD1A, SKD2, VPS4, VPS4-1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-32922 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-32922, RRID:AB_2215497
- Product name
- Anti-VPS4A
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Anti-glycoprotein H antibody impairs the pathogenicity of varicella-zoster virus in skin xenografts in the SCID mouse model.
Vleck SE, Oliver SL, Reichelt M, Rajamani J, Zerboni L, Jones C, Zehnder J, Grose C, Arvin AM
Journal of virology 2010 Jan;84(1):141-52
Journal of virology 2010 Jan;84(1):141-52
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 49
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
