NB100-65535

antibody from Novus Biologicals

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NB100-65535

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-65535, RRID:AB_960359

Product name

Mouse Monoclonal CD44 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified. Specifically recognizes an epitope encoded by exons v7-v8 on the variant portion of human CD44.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of representative areas of synovial tissue samples taken from patients with end-stage osteoarthritis (OA) or refractory rheumatoid arthritis (RA) is shown. Expression pattern and staining intensity of RA samples represent the stronger intensity of synovial inflammation when compared with OA samples.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of activation markers in synovial membranes of osteoarthritis. Phenotypic markers that are instrumental in extracellular matrix alteration are detectable only at a low level (MMP-1, CD44v7/8) in areas that are slightly FAP-positive. FAP, fibroblast activation protein; MMP, matrix metalloproteinase.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of activation markers in synovial membranes of rheumatoid arthritis. Expression of fibroblast activation protein (FAP) is accompanied by accumulation of activation markers (MMP-1 and MMP-13) and splice variants of CD44 (v3 and v7/8) that are known to correlate with the degree of inflammation and that are involved in extracellular matrix deposition.