- HPA005785 - Atlas Antibodies CD44 antibody

Submitted references EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer.

Schneck H, Gierke B, Uppenkamp F, Behrens B, Niederacher D, Stoecklein NH, Templin MF, Pawlak M, Fehm T, Neubauer H, Disseminated Cancer Cell Network (DCC Net) Duesseldorf
PloS one 2015;10(12):e0144535

Superficial scrapings from breast tumors is a source for biobanking and research purposes

Ma R, Fredriksson I, Karthik G, Winn G, Darai-Ramqvist E, Bergh J, Hartman J
Laboratory Investigation 2014 April;94(7):796-805

Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters

Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Némati F, Laval J, Château-Joubert S, Servely J, Vallerand D, Fontaine J, Taylor N, Battini J, Sitbon M, Decaudin D
Laboratory Investigation 2013 March;93(5):611-621

Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay

Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bäuerle T, Wallwiener M, Holland-Letz T, Höfner T, Sprick M, Scharpff M, Marmé F, Sinn H, Pantel K, Weichert W, Trumpp A
Nature Biotechnology 2013 April;31(6):539-544

Metformin regulates breast cancer stem cell ontogeny by transcriptional regulation of the epithelial-mesenchymal transition (EMT) status.

Vazquez-Martin A, Oliveras-Ferraros C, Cufí S, Del Barco S, Martin-Castillo B, Menendez JA
Cell cycle (Georgetown, Tex.) 2010 Sep 15;9(18):3807-14

Synthesis of hyaluronan in oesophageal cancer cells is uncoupled from the prostaglandin-cAMP pathway.

Twarock S, Röck K, Sarbia M, Weber AA, Jänicke RU, Fischer JW
British journal of pharmacology 2009 May;157(2):234-43

Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma

Asplund A, Gry Björklund M, Sundquist C, Strömberg S, Edlund K, Östman A, Nilsson P, Pontén F, Lundeberg J
British Journal of Dermatology 2008 March;158(3):527-538

No comments: Submit comment

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Western blot analysis in human cell lines U-251MG and MCF-7 using Anti-CD44 antibody. Corresponding CD44 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Orthogonal method: Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

: Show more

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human skin and skeletal muscle tissues using HPA005785 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

: Show more

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin shows strong membranous positivity in squamous epithelial cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human breast shows strong membranous positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human squamous cell lung carcinoma shows strong membranous positivity in tumor cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node shows strong positivity in plasma membrane in non-germinal center cells.
Sample type: HUMAN

HPA005785

Antibody data