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LearnMAB4801
antibody from Novus Biologicals
Targeting: IL1RN
ICIL-1RA, IL-1RN, IL1F3, IL1RA, IRAP, MGC10430
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- MAB4801 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IL-1ra/IL-1F3/IL1RN Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-1ra/IL-1F3 in direct ELISAs and Western blots. In Western blots, approximately 30%-50% cross-reactivity with recombinant equine IL-1ra and recombinant human (rh) IL-1ra is observed. Less than 5% cross-reactivity with recombinant mouse (rm) IL-1b, rmIL-18, rmIL-36Ra, rmIL-36a, rmIL-36b, rmIL-1F10, rhIL-36, rhIL-1F7, or recombinant porcine IL-1ra, or recombinant rat IL-1ra is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse IL-1ra/IL-1F3 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line treated with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB4801) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-1ra/IL-1F3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
