Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF-480-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-1ra/IL-1F3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-1ra/IL-1F3 in ELISAs and Western blots. In sandwich ELISAs, approximately 45% cross-reactivity with recombinant rat IL-1ra/IL-1F3 and less than 1% cross-reactivity with recombinant human IL-1ra/IL-1F3, recombinant porcine IL-1ra/IL-1F3, and recombinant equine IL-1ra/IL-1F3 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Effective combinatorial immunotherapy for castration-resistant prostate cancer.
Correction: Yersinia pestis Activates Both IL-1β and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague.
Brain interleukin-1β and the intrinsic receptor antagonist control peripheral Toll-like receptor 3-mediated suppression of spontaneous activity in rats.
Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis.
Interleukin 1 receptor antagonist mediates the antiinflammatory and antifibrotic effect of mesenchymal stem cells during lung injury.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
Sequential ELISA to profile multiple cytokines from small volumes.
Lu X, Horner JW, Paul E, Shang X, Troncoso P, Deng P, Jiang S, Chang Q, Spring DJ, Sharma P, Zebala JA, Maeda DY, Wang YA, DePinho RA
Nature 2017 Mar 30;543(7647):728-732
Nature 2017 Mar 30;543(7647):728-732
Correction: Yersinia pestis Activates Both IL-1β and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague.
PLOS Pathogens Staff.
PLoS pathogens 2015 May;11(5):e1004921
PLoS pathogens 2015 May;11(5):e1004921
Brain interleukin-1β and the intrinsic receptor antagonist control peripheral Toll-like receptor 3-mediated suppression of spontaneous activity in rats.
Yamato M, Tamura Y, Eguchi A, Kume S, Miyashige Y, Nakano M, Watanabe Y, Kataoka Y
PloS one 2014;9(3):e90950
PloS one 2014;9(3):e90950
Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis.
Osuchowski MF, Connett J, Welch K, Granger J, Remick DG
Critical care medicine 2009 May;37(5):1567-73
Critical care medicine 2009 May;37(5):1567-73
Interleukin 1 receptor antagonist mediates the antiinflammatory and antifibrotic effect of mesenchymal stem cells during lung injury.
Ortiz LA, Dutreil M, Fattman C, Pandey AC, Torres G, Go K, Phinney DG
Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 26;104(26):11002-7
Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 26;104(26):11002-7
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S
Neuroscience 2005;132(3):625-31
Neuroscience 2005;132(3):625-31
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1ra/IL-1F3 Inhibition of IL-1 alpha/IL-1F1-dependent Cell Proliferation and Neutralization by Mouse IL-1ra/IL-1F3 Antibody. Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM) inhibits Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL-1ra/IL-1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).
