Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF780 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-1ra/IL-1F3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine IL-1ra/IL-1F3 in direct ELISAs and Western blots.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q29056
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Induction of interleukin-1 in articular cartilage by explantation and cutting.
Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J
Arthritis and rheumatism 2004 Aug;50(8):2539-46
Arthritis and rheumatism 2004 Aug;50(8):2539-46
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1ra/IL-1F3 in Porcine PBMCs. IL-1ra/IL-1F3 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL-1ra/ IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF780) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1ra/IL-1F3 Inhibition of IL-1 alpha/IL-1F1-dependent Cell Proliferation and Neutral-ization by Porcine IL-1ra/ IL-1F3 Antibody. Recombinant Porcine IL-1ra/ IL-1F3 (Catalog # 780-RA) inhibits Recombinant Porcine IL-1 alpha/IL-1F1 (Catalog # 680-PI) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Porcine IL-1 alpha/ IL-1F1 (75 pg/mL) activity elicited by Recombinant Porcine IL-1ra/ IL-1F3 (0.48 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Porcine IL-1ra/IL-1F3 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF780). The ND50 is typically 3-15 µg/mL in the presence of concanavalin A (1.25 µg/mL).