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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31317 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OASL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, HCT116.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OASL was performed by loading 100 ng of recombinant OASL protein (lane 1) cell lysates in RIPA Lysis and Extraction buffer (Product # 89900) and Page Ruler Plus Protein Ladder (Product # 26619) onto a 30% Acrylamide/Bis gel. Proteins were transferred and membrane was blocked in 5% milk for one hour at room temperature. OASL was detected at approximately 59 kDa using an OASL polyclonal antibody (Product # PA5-31317) at a dilution of 1:1000 in 5% milk overnight at at 4°C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for one hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076) and the myECL Imager (Product # 62236). Data courtesy of the Xu Lab University of Wisconsin Madison.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using OASL Polyclonal Antibody (Product # PA5-31317). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. OASL Polyclonal Antibody (Product # PA5-31317) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of OASL in paraformaldehyde-fixed HeLa cells using an OASL polyclonal antibody (Product # PA5-31317) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using OASL (Product # PA5-31317) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
