NBP1-81734

antibody from Novus Biologicals

Targeting: LONP1 hLON, LonHS, PIM1, PRSS15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-81734

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-81734, RRID:AB_11002818

Product name

Rabbit Polyclonal LONP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human LONP1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat, Drosophila

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Concentration of mitochondrial DNA mutations by cytoplasmic transfer from platelets to cultured mouse cells.

Ishikawa K, Kobayashi K, Yamada A, Umehara M, Oka T, Nakada K
PloS one 2019;14(3):e0213283

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Reciprocal Roles of Tom7 and OMA1 during Mitochondrial Import and Activation of PINK1.

Sekine S, Wang C, Sideris DP, Bunker E, Zhang Z, Youle RJ
Molecular cell 2019 Mar 7;73(5):1028-1043.e5

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Lon protease inactivation in Drosophila causes unfolded protein stress and inhibition of mitochondrial translation.

Pareek G, Thomas RE, Vincow ES, Morris DR, Pallanck LJ
Cell death discovery 2018;4:51

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Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPARγ-UCP2-AMPK axis.

Boutoual R, Meseguer S, Villarroya M, Martín-Hernández E, Errami M, Martín MA, Casado M, Armengod ME
Scientific reports 2018 Jan 18;8(1):1163

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Loss of the Drosophila m-AAA mitochondrial protease paraplegin results in mitochondrial dysfunction, shortened lifespan, and neuronal and muscular degeneration.

Pareek G, Thomas RE, Pallanck LJ
Cell death & disease 2018 Feb 21;9(3):304

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microRNA-mediated differential expression of TRMU, GTPBP3 and MTO1 in cell models of mitochondrial-DNA diseases.

Meseguer S, Boix O, Navarro-González C, Villarroya M, Boutoual R, Emperador S, García-Arumí E, Montoya J, Armengod ME
Scientific reports 2017 Jul 24;7(1):6209

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PINK1-Parkin pathway activity is regulated by degradation of PINK1 in the mitochondrial matrix.

Thomas RE, Andrews LA, Burman JL, Lin WY, Pallanck LJ
PLoS genetics 2014;10(5):e1004279

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The accumulation of misfolded proteins in the mitochondrial matrix is sensed by PINK1 to induce PARK2/Parkin-mediated mitophagy of polarized mitochondria.

Jin SM, Youle RJ
Autophagy 2013 Nov 1;9(11):1750-7

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Proteomics analysis reveals novel components in the detergent-insoluble subproteome in Alzheimer's disease.

Gozal YM, Duong DM, Gearing M, Cheng D, Hanfelt JJ, Funderburk C, Peng J, Lah JJ, Levey AI
Journal of proteome research 2009 Nov;8(11):5069-79

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: LONP1 Antibody [NBP1-81734] - Simple Western lane view shows a specific band for LONP1 in 0.2 mg/ml of h. Kidney (left) , NIH-3T3 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: LONP1 Antibody [NBP1-81734] - Electropherogram image(s) of corresponding Simple Western lane view. LONP1 antibody was used at 1:20 dilution on h. Kidney and NIH-3T3 lysate(s).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LONP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LONP1 Antibody [NBP1-81734] - Analysis using Anti-LONP1 antibody NBP1-81734 (A) shows similar pattern to independent antibody NBP2-76504 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LONP1 Antibody [NBP1-81734] - PINK1 accumulates upon knockdown of Lon. Western blot analysis of Lon protease (Lon), Mitochondrial transcription factor A (TFAM), Heat shock protein 60 (Hsp60) and Actin in whole head homogenate from control and Lon deficient animals. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1004279), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human duodenum shows granular cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human fallopian tube shows granular cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human pancreas shows granular cytoplasmic positivity.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human tonsil shows granular cytoplasmic positivity.