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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- A303-821A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse PSMC1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from 293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 µg), Jurkat (J; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMC1 antibody A303-821A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. PSMC1 was not successfully immunoprecipitated by rabbit anti-PSMC1 antibody A303-820A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse PSMC1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 (Cat. No. A303-821A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PSMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 (Cat. No. A303-821A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
