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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-29701 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WWOX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Raji, mouse brain, rat brain, HepG2.
- Concentration
- 1 mg/mL
Submitted references Fragile Gene WWOX Guides TFAP2A/TFAP2C-Dependent Actions Against Tumor Progression in Grade II Bladder Cancer.
In vitro and in silico assessment of the effect of WWOX expression on invasiveness pathways associated with AP-2 transcription factors in bladder cancer.
WWOX Loses the Ability to Regulate Oncogenic AP-2γ and Synergizes with Tumor Suppressor AP-2α in High-Grade Bladder Cancer.
Kołat D, Kałuzińska Ż, Płuciennik E
Frontiers in oncology 2021;11:621060
Frontiers in oncology 2021;11:621060
In vitro and in silico assessment of the effect of WWOX expression on invasiveness pathways associated with AP-2 transcription factors in bladder cancer.
Kałuzińska Ż, Kołat D, Kośla K, Orzechowska M, Bednarek AK, Płuciennik E
BMC urology 2021 Mar 10;21(1):36
BMC urology 2021 Mar 10;21(1):36
WWOX Loses the Ability to Regulate Oncogenic AP-2γ and Synergizes with Tumor Suppressor AP-2α in High-Grade Bladder Cancer.
Kołat D, Kałuzińska Ż, Bednarek AK, Płuciennik E
Cancers 2021 Jun 12;13(12)
Cancers 2021 Jun 12;13(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WWOX was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a WWOX Polyclonal Antibody (Product # PA5-29701) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WWOX Polyclonal Antibody (Product # PA5-29701). Sample (30 µg of whole cell lysate). Lane A: Raji. 12% SDS PAGE. WWOX Polyclonal Antibody (Product # PA5-29701) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WWOX Polyclonal Antibody (Product # PA5-29701). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. WWOX Polyclonal Antibody (Product # PA5-29701) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of WWOX in paraformaldehyde-fixed A549 cells using a WWOX polyclonal antibody (Product # PA5-29701) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WWOX Polyclonal Antibody detects WWOX protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. WWOX Polyclonal Antibody (Product # PA5-29701) dilution: 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WWOX Polyclonal Antibody detects WWOX protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. WWOX Polyclonal Antibody (Product # PA5-29701) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WWOX Polyclonal Antibody immunoprecipitates WWOX protein in IP experiments. IP samples: 293T whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of WWOX protein by 4 µg WWOX Polyclonal Antibody (Product # PA5-29701). 10 % SDS-PAGE. The immunoprecipitated WWOX protein was detected by WWOX Polyclonal Antibody (Product # PA5-29701) diluted at 1:500.
