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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB10681 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10681, RRID:AB_11188575
- Product name
- HIF1A monoclonal antibody, clone 1A3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant HIF1A.
- Isotype
- IgG
- Antibody clone number
- 1A3
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) against COS-7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) .
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (A) , lung cancer tissues (B) , stomach cancer tissues (C) and brain tumor tissues (D) using HIF1A monoclonal antibody, clone 1A3 (Cat # MAB10681) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
