GTX30105

antibody from GeneTex

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Gel shift (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX30105

Provider

GeneTex

Proper citation

GeneTex Cat#GTX30105, RRID:AB_372307

Product name

HIF1 alpha antibody [H1alpha67]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Porcine, Rabbit, Sheep, Simian, Xenopus, Yeast

Host

Mouse

References

Submitted references

Fibrin patch influences the expression of hypoxia-inducible factor-1α and nuclear factor-κBp65 factors on ischemic intestinal anastomosis.

García-Vásquez C, Fernández-Aceñero MJ, García Gómez-Heras S, Pastor C
Experimental biology and medicine (Maywood, N.J.) 2018 Jun;243(10):803-808

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Hypothermic neuroprotections in the brain of an echolocation bat, Hipposideros terasensis.

Lin CL, Hsiao CJ, Hsu CH, Wang SE, Jen PH, Wu CH
Neuroreport 2017 Oct 18;28(15):956-962

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Rapid and efficient generation of neural progenitors from adult bone marrow stromal cells by hypoxic preconditioning.

Mung KL, Tsui YP, Tai EW, Chan YS, Shum DK, Shea GK
Stem cell research & therapy 2016 Oct 7;7(1):146

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TNFalpha induces HIF-1alpha expression through activation of IKKbeta.

Kuo HP, Lee DF, Xia W, Wei Y, Hung MC
Biochemical and biophysical research communications 2009 Nov 27;389(4):640-4

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Hypoxia-induced abrogation of contact-dependent inhibition of rheumatoid arthritis synovial fibroblast proliferation.

Nonomura Y, Mizoguchi F, Suzuki A, Nanki T, Kato H, Miyasaka N, Kohsaka H
The Journal of rheumatology 2009 Apr;36(4):698-705

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Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I.

Catrina SB, Botusan IR, Rantanen A, Catrina AI, Pyakurel P, Savu O, Axelson M, Biberfeld P, Poellinger L, Brismar K
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Aug 1;12(15):4506-14

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

MEF cells were grown on 15 cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HIF-1 alpha Antibody [H1alpha67] (GTX30105) - Detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western Blot: HIF-1 alpha Antibody (H1alpha67) (GTX30105) - Analysis of 50μg cobalt chloride (CoCl2) induced COS-7 nuclear extracts

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation using HIF-1 alpha Antibody [H1alpha67] (GTX30105) - Heavy and light chains are also detected.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of human globlastoma multiforme utilizing GTX30105.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-HIF-1 alpha antibody [H1alpha67] (GTX30105).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-HIF-1 alpha antibody [H1alpha67] (GTX30105).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) (GTX30105) - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.