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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- MAB2342 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2342, RRID:AB_10554156
- Product name
- HIF1A monoclonal antibody, clone ESEE122
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant HIF1A.
- Isotype
- IgG
- Antibody clone number
- ESEE122
- Storage
- Store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Are mast cells implicated in asphyxia?
Markers of mechanical asphyxia: immunohistochemical study on autoptic lung tissues.
Antitumor activity of an oncolytic adenoviral-CD40 ligand (CD154) transgene construct in human breast cancer cells.
Expression of hypoxia-inducible factor-1alpha and its relationship to tumour angiogenesis and cell proliferation in cartilage tumours.
Differential regulation of hypoxia-induced CXCR4 triggering during B-cell development and lymphomagenesis.
Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation.
Hypoxia at the site of abdominal aortic aneurysm rupture is not associated with increased lactate.
Muciaccia B, Sestili C, De Grossi S, Vestri A, Cipolloni L, Cecchi R
International journal of legal medicine 2016 Jan;130(1):153-61
International journal of legal medicine 2016 Jan;130(1):153-61
Markers of mechanical asphyxia: immunohistochemical study on autoptic lung tissues.
Cecchi R, Sestili C, Prosperini G, Cecchetto G, Vicini E, Viel G, Muciaccia B
International journal of legal medicine 2014 Jan;128(1):117-25
International journal of legal medicine 2014 Jan;128(1):117-25
Antitumor activity of an oncolytic adenoviral-CD40 ligand (CD154) transgene construct in human breast cancer cells.
Gomes EM, Rodrigues MS, Phadke AP, Butcher LD, Starling C, Chen S, Chang D, Hernandez-Alcoceba R, Newman JT, Stone MJ, Tong AW
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 15;15(4):1317-25
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 15;15(4):1317-25
Expression of hypoxia-inducible factor-1alpha and its relationship to tumour angiogenesis and cell proliferation in cartilage tumours.
Kubo T, Sugita T, Shimose S, Matsuo T, Arihiro K, Ochi M
The Journal of bone and joint surgery. British volume 2008 Mar;90(3):364-70
The Journal of bone and joint surgery. British volume 2008 Mar;90(3):364-70
Differential regulation of hypoxia-induced CXCR4 triggering during B-cell development and lymphomagenesis.
Piovan E, Tosello V, Indraccolo S, Masiero M, Persano L, Esposito G, Zamarchi R, Ponzoni M, Chieco-Bianchi L, Dalla-Favera R, Amadori A
Cancer research 2007 Sep 15;67(18):8605-14
Cancer research 2007 Sep 15;67(18):8605-14
Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation.
Khan Z, Michalopoulos GK, Stolz DB
The American journal of pathology 2006 Oct;169(4):1251-69
The American journal of pathology 2006 Oct;169(4):1251-69
Hypoxia at the site of abdominal aortic aneurysm rupture is not associated with increased lactate.
Choke E, Cockerill GW, Dawson J, Chung YL, Griffiths J, Wilson RW, Loftus IM, Thompson MM
Annals of the New York Academy of Sciences 2006 Nov;1085:306-10
Annals of the New York Academy of Sciences 2006 Nov;1085:306-10
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Staining of HIF1A (red dye 568) in a cultured Raw 264.7 cell line, using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection of HIF1A (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Staining of HIF1A in hypoxia-induced human placenta using HIF1A monoclonal antibody, clone ESEE122 (Cat #MAB2342).
- Validation comment
- Immunohistochemistry
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Negative control stain of human placenta (from sea level) using mouse IgG at 1 : 100. 4uM paraffin-embedded section.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
