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Learn100-401-A25
antibody from Rockland Immunochemicals, Inc.
Targeting: HIF1A
bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 100-401-A25 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-A25, RRID:AB_2295214
- Product name
- Anti-Hif-1? hydroxy P564 (RABBIT) Antibody - 100-401-A25
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references The evolutionarily conserved arginyltransferase 1 mediates a pVHL-independent oxygen-sensing pathway in mammalian cells.
Moorthy BT, Jiang C, Patel DM, Ban Y, O'Shea CR, Kumar A, Yuan T, Birnbaum MD, Gomes AV, Chen X, Fontanesi F, Lampidis TJ, Barrientos A, Zhang F
Developmental cell 2022 Mar 14;57(5):654-669.e9
Developmental cell 2022 Mar 14;57(5):654-669.e9
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-????The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO. Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
- Validation comment
- Western Blot
