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- References [3]
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- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- GTX28925 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX28925, RRID:AB_371662
- Product name
- NOTCH1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Role of stromal cell-mediated Notch signaling in CLL resistance to chemotherapy.
Notch 1 signaling pathway effect on implantation competency.
Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast.
Nwabo Kamdje AH, Bassi G, Pacelli L, Malpeli G, Amati E, Nichele I, Pizzolo G, Krampera M
Blood cancer journal 2012 May;2(5):e73
Blood cancer journal 2012 May;2(5):e73
Notch 1 signaling pathway effect on implantation competency.
Chu PW, Wang YP, Chen IC, Pan HM, Wu GJ
Fertility and sterility 2011 Nov;96(5):1225-9
Fertility and sterility 2011 Nov;96(5):1225-9
Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast.
Cheng X, Huber TL, Chen VC, Gadue P, Keller GM
Development (Cambridge, England) 2008 Oct;135(20):3447-58
Development (Cambridge, England) 2008 Oct;135(20):3447-58
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot in 293 cells transiently transfected with myc-tagged mouse Notch. Bound antibody was detected with a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122), and color development. Lane M: Mol wt markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc. Personal communication, Dr. Stacey Huppert.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Notch1 antibody (GTX28925). Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17ß-estradiol. Lane 3: MCF-7 + 10 µM gamma secretase inhibitor. Load: 35 µg per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4°C. Secondary antibody: infrared rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80 kDa for Notch1.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Notch1 antibody (GTX28925). Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17?-estradiol. Lane 3: MCF-7 + 10 ?M gamma secretase inhibitor. Load: 35 ?g per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4¢XC. Rabbit secondary antibody at 1:10,000 for 45 min at RT.Predicted/Observed size: 80 kDa for Notch1.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot in 293 cells transiently transfected with myc-tagged mouse Notch. Bound antibody was detected with a 1:10,000 dilution of HRP conjugated Goat anti Rabbit IgG, and color development. Lane M: markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of exocrine glands of human pancreas using Rabbit anti-Notch1 antibody (GTX28925) at a dilution of 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
