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- Antibody Data
- Antigen structure
- References [17]
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- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB219-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-12 p70 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-12 p70 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-12 p40 homodimer or rhIL-23 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 24910
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
IL-1β, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs.
Bacillus Calmette-Guérin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses.
A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes.
The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.
IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.
Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke.
In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model.
Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma.
In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment.
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X
Scientific reports 2016 Sep 2;6:32320
Scientific reports 2016 Sep 2;6:32320
IL-1β, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs.
Bal SM, Bernink JH, Nagasawa M, Groot J, Shikhagaie MM, Golebski K, van Drunen CM, Lutter R, Jonkers RE, Hombrink P, Bruchard M, Villaudy J, Munneke JM, Fokkens W, Erjefält JS, Spits H, Ros XR
Nature immunology 2016 Jun;17(6):636-45
Nature immunology 2016 Jun;17(6):636-45
Bacillus Calmette-Guérin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses.
Suliman S, Geldenhuys H, Johnson JL, Hughes JE, Smit E, Murphy M, Toefy A, Lerumo L, Hopley C, Pienaar B, Chheng P, Nemes E, Hoft DF, Hanekom WA, Boom WH, Hatherill M, Scriba TJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1100-1110
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1100-1110
A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes.
Jones RB, Mueller S, O'Connor R, Rimpel K, Sloan DD, Karel D, Wong HC, Jeng EK, Thomas AS, Whitney JB, Lim SY, Kovacs C, Benko E, Karandish S, Huang SH, Buzon MJ, Lichterfeld M, Irrinki A, Murry JP, Tsai A, Yu H, Geleziunas R, Trocha A, Ostrowski MA, Irvine DJ, Walker BD
PLoS pathogens 2016 Apr;12(4):e1005545
PLoS pathogens 2016 Apr;12(4):e1005545
The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
Ranzani V, Rossetti G, Panzeri I, Arrigoni A, Bonnal RJ, Curti S, Gruarin P, Provasi E, Sugliano E, Marconi M, De Francesco R, Geginat J, Bodega B, Abrignani S, Pagani M
Nature immunology 2015 Mar;16(3):318-325
Nature immunology 2015 Mar;16(3):318-325
Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.
Jo J, Tan AT, Ussher JE, Sandalova E, Tang XZ, Tan-Garcia A, To N, Hong M, Chia A, Gill US, Kennedy PT, Tan KC, Lee KH, De Libero G, Gehring AJ, Willberg CB, Klenerman P, Bertoletti A
PLoS pathogens 2014 Jun;10(6):e1004210
PLoS pathogens 2014 Jun;10(6):e1004210
IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.
Blom L, Poulsen LK
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4331-7
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4331-7
Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Makino H, Ranjan P, Minakuchi K, Sone S
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):8176-85
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):8176-85
Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
The Journal of experimental medicine 2009 Apr 13;206(4):793-805
The Journal of experimental medicine 2009 Apr 13;206(4):793-805
Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
Miller R, Sadhukhan R, Wu C
Journal of immunological methods 2008 May 20;334(1-2):134-41
Journal of immunological methods 2008 May 20;334(1-2):134-41
Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke.
Mian MF, Lauzon NM, Stämpfli MR, Mossman KL, Ashkar AA
Journal of leukocyte biology 2008 Mar;83(3):774-84
Journal of leukocyte biology 2008 Mar;83(3):774-84
In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
The Journal of clinical investigation 2007 Dec;117(12):3868-78
The Journal of clinical investigation 2007 Dec;117(12):3868-78
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L
Blood 2004 Nov 15;104(10):3267-75
Blood 2004 Nov 15;104(10):3267-75
The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model.
Weigt H, Muhlradt PF, Larbig M, Krug N, Braun A
Journal of immunology (Baltimore, Md. : 1950) 2004 May 15;172(10):6080-6
Journal of immunology (Baltimore, Md. : 1950) 2004 May 15;172(10):6080-6
Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma.
Radstake TR, Roelofs MF, Jenniskens YM, Oppers-Walgreen B, van Riel PL, Barrera P, Joosten LA, van den Berg WB
Arthritis and rheumatism 2004 Dec;50(12):3856-65
Arthritis and rheumatism 2004 Dec;50(12):3856-65
In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment.
Durali D, de Goër de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y
Blood 2003 Dec 1;102(12):4084-9
Blood 2003 Dec 1;102(12):4084-9
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-12 p70 in Human Kidney. IL-12 p70 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human IL-12 p70 Monoclonal Antibody (Catalog # MAB219) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-12 and Neutralization by Human IL-12 p70 Antibody. Recombinant Human IL-12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12 p70 Monoclonal Antibody (Catalog # MAB219). The ND50 is typically 0.3-0.9 µg/mL.
