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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [20]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-219-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-12 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse IL-12 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P29459 (p35) & P29460 (p40)- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mycobacterium bovis Bacillus Calmette-Guérin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
Upregulated IL-23 and IL-17 in Behçet patients with active uveitis.
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
Innate immune responses to herpes simplex virus type 2 influence skin homing molecule expression by memory CD4+ lymphocytes.
Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA.
APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA.
NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
Activation of a subset of human NK cells upon contact with Plasmodium falciparum-infected erythrocytes.
Activation of a subset of human NK cells upon contact with Plasmodium falciparum-infected erythrocytes.
Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma.
Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
International immunology 2008 Oct;20(10):1321-9
International immunology 2008 Oct;20(10):1321-9
Upregulated IL-23 and IL-17 in Behçet patients with active uveitis.
Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
Investigative ophthalmology & visual science 2008 Jul;49(7):3058-64
Investigative ophthalmology & visual science 2008 Jul;49(7):3058-64
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
European journal of immunology 2008 Apr;38(4):1012-23
European journal of immunology 2008 Apr;38(4):1012-23
Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5284-92
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5284-92
Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5284-92
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5284-92
Innate immune responses to herpes simplex virus type 2 influence skin homing molecule expression by memory CD4+ lymphocytes.
Koelle DM, Huang J, Hensel MT, McClurkan CL
Journal of virology 2006 Mar;80(6):2863-72
Journal of virology 2006 Mar;80(6):2863-72
Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
Nagarajan S, Selvaraj P
Vaccine 2006 Mar 20;24(13):2264-74
Vaccine 2006 Mar 20;24(13):2264-74
Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
Takei M, Umeyama A, Arihara S, Matsumoto H
Journal of pharmaceutical sciences 2005 May;94(5):974-82
Journal of pharmaceutical sciences 2005 May;94(5):974-82
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5213-21
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5213-21
Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5213-21
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5213-21
APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA.
Schmidt KN, Leung B, Kwong M, Zarember KA, Satyal S, Navas TA, Wang F, Godowski PJ
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):138-43
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):138-43
APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA.
Schmidt KN, Leung B, Kwong M, Zarember KA, Satyal S, Navas TA, Wang F, Godowski PJ
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):138-43
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):138-43
NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7
NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7
Activation of a subset of human NK cells upon contact with Plasmodium falciparum-infected erythrocytes.
Artavanis-Tsakonas K, Eleme K, McQueen KL, Cheng NW, Parham P, Davis DM, Riley EM
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5396-405
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5396-405
Activation of a subset of human NK cells upon contact with Plasmodium falciparum-infected erythrocytes.
Artavanis-Tsakonas K, Eleme K, McQueen KL, Cheng NW, Parham P, Davis DM, Riley EM
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5396-405
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5396-405
Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
Hessian PA, Highton J, Kean A, Sun CK, Chin M
Arthritis and rheumatism 2003 Feb;48(2):334-8
Arthritis and rheumatism 2003 Feb;48(2):334-8
Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma.
Nutku E, Gounni AS, Olivenstein R, Hamid Q
The Journal of allergy and clinical immunology 2000 Aug;106(2):288-92
The Journal of allergy and clinical immunology 2000 Aug;106(2):288-92
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-12 and Neutralization by Human IL-12 Antibody. Recombinant Human IL-12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND50 is typically 0.03-0.05 µg/mL.
