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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX104717 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104717, RRID:AB_1241426
- Product name
- UBE2L3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
Submitted references The Atypical Ubiquitin E2 Conjugase UBE2L3 Is an Indirect Caspase-1 Target and Controls IL-1β Secretion by Inflammasomes.
Resources for the Comprehensive Discovery of Functional RNA Elements.
Eldridge MJG, Sanchez-Garrido J, Hoben GF, Goddard PJ, Shenoy AR
Cell reports 2017 Jan 31;18(5):1285-1297
Cell reports 2017 Jan 31;18(5):1285-1297
Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- UBE2L3 antibody detects UBE2L3 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract15 % SDS-PAGEUBE2L3 antibody (GTX104717) dilution: 1:1000
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 (GTX27900) 12% SDS PAGE GTX104717 diluted at 1:1000
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- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole zebrafish extract (30 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3(GTX104717) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 £gg of UBE2L3 antibody (GTX104717).Western blot analysis was performed using UBE2L3 antibody (GTX104717).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3(GTX104717) antibody at 1:100 dilution.
