PA5-21598

antibody from Invitrogen Antibodies

Targeting: UBE2L3 UBCH7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21598

Provider

Invitrogen Antibodies

Product name

UBE2L3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: HeLa, HepG2, Neuro2A, GL261, C8D30, mouse testis, whole zebrafish.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of UBE2L3 was performed by separating 50 µg of mouse tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a UBE2L3 Polyclonal Antibody (Product # PA5-21598) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using UBE2L3 Polyclonal Antibody (Product # PA5-21598). Sample (30 µg of whole cell lysate). Lane A: Hela . Lane B: Hep G2. 12% SDS PAGE. UBE2L3 Polyclonal Antibody (Product # PA5-21598) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using UBE2L3 Polyclonal Antibody (Product # PA5-21598). Whole zebrafish extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 Polyclonal Antibody (Product # PA5-21598) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

UBE2L3 Polyclonal Antibody detects UBE2L3 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract.15 % SDS-PAGE. UBE2L3 Polyclonal Antibody (Product # PA5-21598) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Ube2L3 in paraformaldehyde-fixed HeLa cells using a Ube2L3 polyclonal antibody (Product # PA5-21598) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3 (Product # PA5-21598) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3 (Product # PA5-21598) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.