Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-30995 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-30995, RRID:AB_2304029
- Product name
- Rabbit Polyclonal UbcH7/UBE2L3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract, C. 30 ug C8D30 whole cell lysate/extract 15 % SDS-PAGE gel, antibody dilution 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody (GTX104717).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody (GTX104717).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: UbcH7/UBE2L3 Antibody [NBP1-30995] - Immunoprecipitation of UbcH7/UBE2L3 protein from HeLa whole cell extracts using 5 ug of UbcH7/UBE2L3 antibody (NBP1-30995). Western blot analysis was performed using UbcH7/UBE2L3 antibody (NBP1-30995). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP1-30995] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.