PA5-22383

antibody from Invitrogen Antibodies

Targeting: NPEPPS MP100, PSA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22383

Provider

Invitrogen Antibodies

Product name

NPEPPS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, HeLa, HepG2, HCT116, mouse brain.

Concentration

0.68 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NPEPPS using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NPEPPS polyclonal antibody (Product # PA5-22383) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NPEPPS using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NPEPPS polyclonal antibody (Product # PA5-22383) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NPEPPS Polyclonal Antibody (Product # PA5-22383). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS Polyclonal Antibody (Product # PA5-22383) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NPEPPS Polyclonal Antibody (Product # PA5-22383). Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS Polyclonal Antibody (Product # PA5-22383) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NPEPPS in paraformaldehyde-fixed HeLa cells using a NPEPPS polyclonal antibody (Product # PA5-22383) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NPEPPS Polyclonal Antibody detects NPEPPS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NPEPPS stained by NPEPPS Polyclonal Antibody (Product # PA5-22383) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.