Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
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Validation data
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- Product number
- PA5-22383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPEPPS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, mouse brain. Predicted reactivity: Mouse (97%), Rat (97%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NPEPPS using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NPEPPS polyclonal antibody (Product # PA5-22383) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NPEPPS using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NPEPPS polyclonal antibody (Product # PA5-22383) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NPEPPS Polyclonal Antibody (Product # PA5-22383). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS Polyclonal Antibody (Product # PA5-22383) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NPEPPS Polyclonal Antibody (Product # PA5-22383). Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS Polyclonal Antibody (Product # PA5-22383) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NPEPPS in paraformaldehyde-fixed HeLa cells using a NPEPPS polyclonal antibody (Product # PA5-22383) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NPEPPS Polyclonal Antibody detects NPEPPS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NPEPPS stained by NPEPPS Polyclonal Antibody (Product # PA5-22383) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.