MA5-47084

antibody from Invitrogen Antibodies

Targeting: NPEPPS MP100, PSA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-47084

Provider

Invitrogen Antibodies

Product name

NPEPPS Monoclonal Antibody (HL1531)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Store as concentrated solution.

Antibody clone number

HL1531

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NPEPPS in Various whole cell extracts (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with NPEPPS Monoclonal antibody (Product # MA5-47084) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NPEPPS in Whole zebrafish extract (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with NPEPPS Monoclonal antibody (Product # MA5-47084) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NPEPPS in HeLa cells. Cells were fixed in ice-cold MeOH for 5 min. Green: NPEPPS stained by NPEPPS monoclonal antibody (Product # MA5-47084) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NPEPPS in paraffin-embedded mouse kidney. Samples were incubated in NPEPPS Monoclonal antibody (Product # MA5-47084). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NPEPPS in paraffin-embedded mouse kidney. Samples were incubated in NPEPPS Monoclonal antibody (Product # MA5-47084). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.