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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- BS2552 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS2552, RRID:AB_1663594
- Product name
- Prothrombin (G724) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 701-750 of Human Prothrombin.
- Description
- Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of Prothrombin (G724) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysateLane3:PC12 whole cell lysate
