PAB28545

antibody from Abnova Corporation

Targeting: A2M CPAMD5, FWP007, S863-7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28545

Provider

Abnova Corporation

Product name

A2M polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant A2M.

Antigen sequence

SVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQ
DDEDCINRHNVYINGITYTPVSSTNEKDMYSFLED
MGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGF
YESDVMGRGHARLVHVEEPHTETVRKYFPETWIWD
LVVVNS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with A2M polyclonal antibody (PAB28545).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of U373 MG with A2M polyclonal antibody (Cat # PAB28545) at 1-4 ug/mL dilution shows positivity in nucleoli & nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human liver with A2M polyclonal antibody (Cat # PAB28545) shows moderate cytoplasmic positivity in hepatocytes at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)