66126-1-IG

antibody from Invitrogen Antibodies

Targeting: A2M CPAMD5, FWP007, S863-7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66126-1-IG

Provider

Invitrogen Antibodies

Product name

Alpha-2-macroglobulin Monoclonal Antibody (4B11F7)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: KDNGCFRSS GSLLNNAIKG GVEDEVTLSA YITIALLEIP LTVTHPVVRN ALFCLESAWK TAQEGDHGSH VYTKALLAYA FALAGNQDKR KEVLKSLNEE AVKKDNSVHW ERPQKPKAPV GHFYEPQAPS AEVEMTSYVL LAYLTAQPAP TSEDLTSATN IVKWITKQQN AQGGFSSTQD TVVALHALSK YGAATFTRTG KAAQVTIQSS GTFSSKFQVD NNNRLLLQQV SLPELPGEYS MKVTGEGCVY LQTSLKYNIL PEKEEFPFAL GVQTLPQTCD EPKAHTSFQI SLSVSYTGSR SASNMAIVDV KMVSGFIPLK PTVKMLERSN HVSRTEVSSN HVLIYLDKVS NQTLSLFFTV LQDVPVRDLK PAIVKVYDYY ETDEFAIAEY NAPCSKDLGN A (1075-1474 aa encoded by BC040071)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4B11F7

Vial size

150 µL

Concentration

1.8 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 66126-1-IG (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 66126-1-IG (Alpha-2-macroglobulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver slide using 66126-1-IG ( Alpha-2-macroglobulin antibody at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver slide using 66126-1-IG ( Alpha-2-macroglobulin antibody at dilution of 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Alpha-2-macroglobulin (IP:66126-1-IG, 5ug; Detection:66126-1-IG 1:500) with human plasma tissue lysate 4000ug.