Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-301-388 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-388, RRID:AB_2201636
- Product name
- Anti-Thyroglobulin (MOUSE) Monoclonal Antibody - 200-301-388
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using ROCKLAND Immunochemical's Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 ?g, right lane 3.0 ?g) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
- Validation comment
- Western Blot