H00002161-M01

antibody from Abnova Corporation

Targeting: F12

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002161-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002161-M01, RRID:AB_463909

Product name

F12 monoclonal antibody (M01), clone 3A3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant F12.

Antigen sequence

GHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHGS
SILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAE
RRLTLQGIISWGSGCGDRNKPGVYTDVAYYLAWIR
EHTVS


Isotype

IgG

Antibody clone number

3A3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged F12 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between GP1BA and F12. HeLa cells were stained with anti-GP1BA rabbit purified polyclonal 1:1200 and anti-F12 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)