66089-1-IG

antibody from Invitrogen Antibodies

Targeting: F12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66089-1-IG

Provider

Invitrogen Antibodies

Product name

Factor XII Monoclonal Antibody (2E2F11)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MPAQPAPPK PQPTTRTPPQ SQTPGALPAK REQPPSLTRN GPLSCGQRLR KSLSSMTRVV GGLVALRGAH PYIAALYWGH SFCAGSLIAP CWVLTAAHCL QDRPAPEDLT VVLGQERRNH SCEPCQTLAV RSYRLHEAFS PVSYQHDLAL LRLQEDADGS CALLSPYVQP VCLPSGAARP SETTLCQVAG WGHQFEGAEE YASFLQEAQV PFLSLERCSA PDVHGSSILP GMLCAGFLEG GTDACQGDSG GPLVCEDQAA ERRLTLQGII SWGSGCGDRN KPGVYTDVAY YLAWIREHTV S (316-615 aa encoded by BC012390)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

2E2F11

Vial size

150 µL

Concentration

1.28 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-IG (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 66089-1-IG (Factor XII antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using F12 antibody 66089-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66089-1-IG ( Factor XII antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66089-1-IG ( Factor XII antibody at dilution of 1:200 (under 40x lens).