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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-54506 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ANKIB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QDPNINDNLL GNIMAWFHDM NPQSIALIPP ATTEISADSQ LPCIKDGSEG VKDVELVLPE DSMFEDASVS EGRGTQIEEN PLEENILAGE AASQAGDSGN EAANRGDGSD VSSQTPQTSS DWLEQV
- Concentration
- 0.2 mg/mL
Submitted references The chlamydial deubiquitinase Cdu1 supports recruitment of Golgi vesicles to the inclusion.
Auer D, Hügelschäffer SD, Fischer AB, Rudel T
Cellular microbiology 2020 May;22(5):e13136
Cellular microbiology 2020 May;22(5):e13136
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ANKIB1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using an ANKIB1 Polyclonal Antibody (Product # PA5-54506).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ANKIB1 in human cell line A-431 shows positivity in cytoplasm. Samples were probed using an ANKIB1 Polyclonal Antibody (Product # PA5-54506).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ANKIB1 in human cell line U-2 OS using an ANKIB1 Polyclonal Antibody (Product # PA5-54506) shows localization to plasma membrane and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ANKIB1 in human testis tissue shows strong cytoplasmic and nuclear positivity in cells of the seminiferus ducts. Samples were probed using an ANKIB1 Polyclonal Antibody (Product # PA5-54506).
