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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V2136 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HSP60 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant human HSP60 was used as the immunogen for this antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- LK1
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references MUTYH, an adenine DNA glycosylase, mediates p53 tumor suppression via PARP-dependent cell death.
Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease.
S-guanylation proteomics for redox-based mitochondrial signaling.
Synergistic and differential modulation of immune responses by Hsp60 and lipopolysaccharide.
Circulating human heat shock protein 60 in the plasma of British civil servants: relationship to physiological and psychosocial stress.
Self-heat shock protein 60 induces tumour necrosis factor-alpha in monocyte-derived macrophage: possible role in chronic inflammatory periodontal disease.
Fibronectin-binding protein A of Staphylococcus aureus has multiple, substituting, binding regions that mediate adherence to fibronectin and invasion of endothelial cells.
A role of Hsp60 in autoimmune diabetes: analysis in a transgenic model.
Oka S, Leon J, Tsuchimoto D, Sakumi K, Nakabeppu Y
Oncogenesis 2014 Oct 13;3:e121
Oncogenesis 2014 Oct 13;3:e121
Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease.
Noelker C, Morel L, Osterloh A, Alvarez-Fischer D, Lescot T, Breloer M, Gold M, Oertel WH, Henze C, Michel PP, Dodel RC, Lu L, Hirsch EC, Hunot S, Hartmann A
Journal of neuroinflammation 2014 May 8;11:86
Journal of neuroinflammation 2014 May 8;11:86
S-guanylation proteomics for redox-based mitochondrial signaling.
Rahaman MM, Sawa T, Ahtesham AK, Khan S, Inoue H, Irie A, Fujii S, Akaike T
Antioxidants & redox signaling 2014 Jan 10;20(2):295-307
Antioxidants & redox signaling 2014 Jan 10;20(2):295-307
Synergistic and differential modulation of immune responses by Hsp60 and lipopolysaccharide.
Osterloh A, Kalinke U, Weiss S, Fleischer B, Breloer M
The Journal of biological chemistry 2007 Feb 16;282(7):4669-80
The Journal of biological chemistry 2007 Feb 16;282(7):4669-80
Circulating human heat shock protein 60 in the plasma of British civil servants: relationship to physiological and psychosocial stress.
Lewthwaite J, Owen N, Coates A, Henderson B, Steptoe A
Circulation 2002 Jul 9;106(2):196-201
Circulation 2002 Jul 9;106(2):196-201
Self-heat shock protein 60 induces tumour necrosis factor-alpha in monocyte-derived macrophage: possible role in chronic inflammatory periodontal disease.
Ueki K, Tabeta K, Yoshie H, Yamazaki K
Clinical and experimental immunology 2002 Jan;127(1):72-7
Clinical and experimental immunology 2002 Jan;127(1):72-7
Fibronectin-binding protein A of Staphylococcus aureus has multiple, substituting, binding regions that mediate adherence to fibronectin and invasion of endothelial cells.
Massey RC, Kantzanou MN, Fowler T, Day NP, Schofield K, Wann ER, Berendt AR, Höök M, Peacock SJ
Cellular microbiology 2001 Dec;3(12):839-51
Cellular microbiology 2001 Dec;3(12):839-51
A role of Hsp60 in autoimmune diabetes: analysis in a transgenic model.
Birk OS, Douek DC, Elias D, Takacs K, Dewchand H, Gur SL, Walker MD, van der Zee R, Cohen IR, Altmann DM
Proceedings of the National Academy of Sciences of the United States of America 1996 Feb 6;93(3):1032-7
Proceedings of the National Academy of Sciences of the United States of America 1996 Feb 6;93(3):1032-7
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa cell lysate with HSP60 antibody (clone LK1).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP60 antibody (green, clone LK1) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin/paraffin human breast carcinoma stained with HSP60 antibody
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE pancreas tissue with HSP60 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of PFA-fixed human HeLa cells with HSP60 antibody (clone LK1); Red=isotype control, Blue= HSP60 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free HSP60 Antibody (clone LK1). Confirmation of Integrity and Purity of the Antibody.
