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LearnAP09366PU-N
antibody from Acris Antibodies GmbH
Targeting: PPP1R13B
ASPP1, KIAA0771, p53BP2-like, p85
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP09366PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09366PU-N, RRID:AB_2035871
- Product name
- anti PPP1R13B
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to an internal sequence of human ASPP1
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.3 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
