Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB110-40684 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40684, RRID:AB_2237454
- Product name
- Rabbit Polyclonal ASPP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASPP1 Antibody [NB110-40684] - Detection of Human ASPP1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASPP1 antibody NB110-40684 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASPP1 Antibody [NB110-40684] - Detection of Human ASPP1 on HeLa whole cell lysates using NB110-40684. ASPP1 was also immunoprecipitated by another rabbit anti-ASPP1 antibody and NB110-40683.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ASPP1 Antibody [NB110-40684] - Detection of human ASPP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASPP1 antibody NB110-40684 used for IP at 6 ug per reaction. ASPP1 was also immunoprecipitated by rabbit anti-ASPP1 antibody NB110-40683. For blotting immunoprecipitated ASPP1, NB110-40684 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.