PAB10313

antibody from Abnova Corporation

Targeting: AHSG A2HS, FETUA, HSGA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10313

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10313, RRID:AB_1671766

Product name

AHSG polyclonal antibody

Antibody type

Polyclonal

Description

Goat polyclonal antibody raised against a recombinant AHSG.

Storage

Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The serum protein alpha 2-Heremans-Schmid glycoprotein/fetuin-A is a systemically acting inhibitor of ectopic calcification.

Schafer C, Heiss A, Schwarz A, Westenfeld R, Ketteler M, Floege J, Muller-Esterl W, Schinke T, Jahnen-Dechent W
The Journal of clinical investigation 2003 Aug;112(3):357-66

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Interactions of alpha2-HS-glycoprotein (fetuin) with MMP-3 and murine squamous cell carcinoma cells.

Leite-Browning ML, McCawley LJ, Choi OH, Matrisian LM, Ochieng J
International journal of oncology 2002 Nov;21(5):965-71

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Changes with age of the rat fetuin concentration in serum and its mRNA expression.

Kazi JA, Nakamura O, Ohnishi T, Arakaki N, Kajihara T, Nakagawa S, Daikuhara Y
Journal of biochemistry 1998 Jul;124(1):179-86

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoblot of AHSG polyclonal antibody (Cat # PAB10313), generated by immunization with mature protein, was tested by immunoblot against AHSG in purified preparations and in human plasma.Lane 1 contains 250 ng of purified human AHSG.Lane 2 contains 5 uL of a 1 : 50 dilution of human serum.Dilution of Anti-human AHSG antibody between 1 : 10,000 and 1 : 20,000 showed strong reactivity by immunoblot.In this blot the antibody was used at a 1 : 10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS.Detection occurred using a 1 : 5,000 dilution of IRDye™800 conjugated Donkey anti-Goat IgG for 45 min at room temperature.LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.