NBP2-14951

antibody from Novus Biologicals

Targeting: ETS2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-14951

Provider

Novus Biologicals

Product name

Rabbit Polyclonal ETS2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ETS2 Antibody [NBP2-14951] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE; antibody diluted at 1:1000.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: ETS2 Antibody [NBP2-14951] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 ug of ETS2 antibody 7.5% SDS-PAGE The immunoprecipitated ETS2 protein was detected by ETS2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ETS2 Antibody [NBP2-14951] - Rat fore brain. ETS2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation: ETS2 Antibody [NBP2-14951] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PTHrP promoter.