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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX104527 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104527, RRID:AB_11179034
- Product name
- ETS2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references Functional dissection of breast cancer risk-associated TERT promoter variants.
Super-Enhancers Promote Transcriptional Dysregulation in Nasopharyngeal Carcinoma.
Helbig S, Wockner L, Bouendeu A, Hille-Betz U, McCue K, French JD, Edwards SL, Pickett HA, Reddel RR, Chenevix-Trench G, Dörk T, Beesley J
Oncotarget 2017 Sep 15;8(40):67203-67217
Oncotarget 2017 Sep 15;8(40):67203-67217
Super-Enhancers Promote Transcriptional Dysregulation in Nasopharyngeal Carcinoma.
Yuan J, Jiang YY, Mayakonda A, Huang M, Ding LW, Lin H, Yu F, Lu Y, Loh TKS, Chow M, Savage S, Tyner JW, Lin DC, Koeffler HP
Cancer research 2017 Dec 1;77(23):6614-6626
Cancer research 2017 Dec 1;77(23):6614-6626
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 10% SDS PAGE GTX104527 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ETS2(GTX104527) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ETS2 antibody immunoprecipitates ETS2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 £gg of ETS2 antibody (GTX104527) 7.5% SDS-PAGE The immunoprecipitated ETS2 protein was detected by ETS2 antibody (GTX104527) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ETS2 antibody detects ETS2 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. ETS2 antibody (GTX104527) dilution: 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 £gg of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PTHrP promoter.
